50 SARS-CoV–dos peptides was chose for each and every of top very prevalent HLA alleles inside the Italy. The selection try based mostly toward SARS-CoV–2 epitopes which had the best forecast binding affinity towards the MHC centered on NetMHCpan–cuatro.016, and finding a prediction rating more than 0.5 using NetChop–step three.117. The latest SARS-CoV–dos proteome are obtained from UniProt (Proteome ID: UP000464024). Thirteen healthy protein (pp1ab (ORF1ab), healthy protein 3a, non-architectural protein 6, protein 7a, non-architectural proteins 7b, non-architectural protein 8, necessary protein 9b, ORF10 necessary protein, uncharacterized proteins 14, envelope short membrane proteins (E), membrane healthy protein (M), nucleoprotein (N) and you can spike glycoprotein (S)) had been thought to be resources of possible epitopes.

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